Device

Part:BBa_K797004

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-20)
Revision as of 23:53, 20 September 2012 by Registry (Talk | contribs)

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Tat secretion cassette with constitutive promoter.

This cassette allow E.coli to secrete proteins with torA signal. Wild type Tat protein secretion system is too week so that Kyoto 2012 construct Tat cassette to reinforce the ability of transportation of Tat system. This parts include tatA,B,C protein coding region and pspA (phage shock protein A). Tat A,B,C protein is the main component of Tat complex where proteins with torA signal go through and pspA can encourage protein secretion via Tat system. Kyoto 2012 suggest iGEMers with this new way of secretion and provide them with this cassette regulated by constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2621
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2048
    Illegal SapI.rc site found at 2081


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Categories
Parameters
None