![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K797004
Designed by: Tomohiro Nobeyama Group: iGEM12_Kyoto (2012-09-20)
Tat secretion cassette with constitutive promoter.
This cassette allow E.coli to secrete proteins with torA signal. Wild type Tat protein secretion system is too week so that Kyoto 2012 construct Tat cassette to reinforce the ability of transportation of Tat system. This parts include tatA,B,C protein coding region and pspA (phage shock protein A). Tat A,B,C protein is the main component of Tat complex where proteins with torA signal go through and pspA can encourage protein secretion via Tat system. Kyoto 2012 suggest iGEMers with this new way of secretion and provide them with this cassette regulated by constitutive promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2621
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2291
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2048
Illegal SapI.rc site found at 2081
[edit]
Categories
Parameters
None |