Generator

Part:BBa_K819008:Design

Designed by: ZHANG Zidong   Group: iGEM12_Peking   (2012-09-03)
Revision as of 08:50, 25 September 2012 by Zhangzidong (Talk | contribs) (→‎Design Notes)

Lux Operon under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


Design Notes

Lux CDABEG genes and related RBS from BBa_K325909 are placed under T7 promoter BBa_K712074.

Source

T7 promoter and LuxCDABE with their RBS are from Partsregistry.

References