Generator

Part:BBa_K819008

Designed by: ZHANG Zidong   Group: iGEM12_Peking   (2012-09-03)
Revision as of 14:17, 3 September 2012 by Registry (Talk | contribs)

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Lux Operon under T7 promoter

luxABCDE gene ligated to the rear of T7 promoter. When transformed into BL21(DE3) E.coli strain, add different amount IPTG to reach different final concentration of IPTG in the medium can induce different level of Lux ligt emission. No substrate is needed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/ecoli
//function/reporter/light
//plasmid/expression
Parameters
emission485nm
n/aLux Operon under T7 promoter
originVibrio.fischeri