Coding
tctB_197aa

Part:BBa_K808005

Designed by: Valentina Herbring, Sebastian Palluk, Andreas Schmidt   Group: iGEM12_TU_Darmstadt   (2012-09-05)
Revision as of 11:55, 24 September 2012 by A.Schmidt (Talk | contribs)

tctB_197: small subunit B2 of the tripartite tricarboxylate transporter family

The small subunit B1 of the tripartite tricarboxylate transporter family (tctB_197, 20,44 kDa) was isolated from Comamonas testosteroni KF-1. The tripartite tricarboxylate transporter system consists of three different proteins: a periplasmatic solute binding receptor, a membrane protein with 12 putative transmembrane alpha-helical spanners (in this case tctB_197), and a small poorly conserved membrane proteine with four putative transmembrane alpha-helical spanners.[1] The strain was purchased from Leibniz Institute DMSZ-German Collection of Microorganism and Cell Cultures (DMSZ no. 14576). To characterized the structure of the tctB_197 bioinformatic tools like Protein Homology/anologY Recognition Engine V 2.0 (PHYRE2), I-TASSER servers, and TMHMM was used. The TMHMM predicted a transmembrane protein with 4 alpha-helical spanners (Fig. 1). The NCBI Protein BLAST results shows that the tctB_197 subunit B2 belongs to the tctB superfamily. Phyre2 an I-Tasser server homology modelling did not give a significant result for the structure of the tctB_197 subunit B2.

Figure 1. TMHMM prediction of the tctB_197 subunit B1. It shows 4 alpha-helical spanners.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345

PHYRE2 - http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index Protein structure prediction on the web: a case study using the PhyreKelley LA and Sternberg MJE.Nature Protocols 4, 363 - 371 (2009 server

I-TaSSER server - http://zhanglab.ccmb.med.umich.edu/I-TASSER/ Yang Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, vol 9, 40 (2008). 

Ambrish Roy, Alper Kucukural, Yang Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, vol 5, 725-738 (2010).

Ambrish Roy, Jianyi Yang, Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, doi:10.1093/nar/gks372 (2012)

http://www.cbs.dtu.dk/services/TatP/ - Prediction of Twin-arginine signal peptides

Prediction of twin-arginine signal peptides. Jannick Dyrløv Bendtsen, Henrik Nielsen, David Widdick, Tracy Palmer and Søren Brunak. BMC bioinformatics 2005 6: 167.

http://www.cbs.dtu.dk/services/SignalP/ - Prediction of Signal peptide and cleavage sites in gram+, gram- and eukaryotic amino acid sequences

SignalP 4.0: discriminating signal peptides from transmembrane regions Thomas Nordahl Petersen, Søren Brunak, Gunnar von Heijne & Henrik Nielsen Nature Methods, 8:785-786, 2011

http://www.cbs.dtu.dk/services/TMHMM/ - Prediciton of transmembrane helices in proteins

Erik L.L. Sonnhammer, Gunnar von Heijne, and Anders Krogh:A hidden Markov model for predicting transmembrane helices in protein sequences. In Proc. of Sixth Int. Conf. on Intelligent Systems for Molecular Biology, p 175-182,Ed J. Glasgow, T. Littlejohn, F. Major, R. Lathrop, D. Sankoff, and C. Sensen Menlo Park, CA: AAAI Press, 1998

http://www.sciencegateway.org/tools/proteinmw.htm - Protein Molecular Weight Caltulator


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