Reporter

Part:BBa_K864120

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-25)
Revision as of 06:50, 25 September 2012 by Registry (Talk | contribs)

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pMB1 ori - RFP cassette


pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to the standard RFP casette BBa_J04450. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pMB1ori-red".

LacI sensitive CAP sensitive

Usage and Biology

Usable as default insert of low and medium copy backbones. This is the default insert of the pSB4x15 series of backbones.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1463
    Illegal AgeI site found at 1575
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None