Composite
phaC1-A-B1

Part:BBa_K934001

Designed by: Taku Nakayama   Group: iGEM12_Tokyo_Tech   (2012-09-17)
Revision as of 05:05, 25 September 2012 by Liu (Talk | contribs)

phaC1-A-B1 [P(3HB) synthesis]

The sequence comes from Ralstonia eutropha H16 adapted to become a standard BioBrick part.

Poly-3-hydroxybutyrate, P(3HB) is synthesized by three enzymes synthesized from phaC1，phaA，phaB1，respectively.

The A1 gene encodes for the 393 amino acids protein, 3-ketothiolase (PhaA)

The B gene encodes for the 246 amino acids protein, acetoacetyl-CoA reductase (PhaB)

The C1 gene encodes for the 589 amino acids protein, PHA Synthase (PhaC)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 916
    Illegal BglII site found at 1741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal NgoMIV site found at 293
    Illegal NgoMIV site found at 893
    Illegal NgoMIV site found at 1205
    Illegal NgoMIV site found at 1484
    Illegal NgoMIV site found at 2136
    Illegal NgoMIV site found at 2158
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4002


[edit]
Categories
Parameters
None