Part:BBa_K934001:Experience

phaC1-A-B1 [P(3HB) synthesis]

FIG1 is the photographs of E.coli colonies on Nile Red positive medium taken under UV. The orange colonies in FIG1.a show that the accumulated PHB in cells was stained by Nile red. This result indicates that part BBa_K934001 synthesized PHB. FIG1.b is the photograph of negative control cells. In this figure we observed that there were no remarkable colored colonies. FIG2 shows the difference between cells storing PHB and those not storing PHB. The cells in blue rectangle area are the cells with PHB synthesis gene and the cells in green rectangle area are the cells with plasI-gfp gene as a negative control. Using the cells storing PHB, we drew a rose silhouette on the LB agar plate containing Nile red. (FIG3).

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