Coding
d6des

Part:BBa_K925003

Designed by: Veronica Rezelj   Group: iGEM12_St_Andrews   (2012-09-24)
Revision as of 23:08, 24 September 2012 by Registry (Talk | contribs)

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Delta-6 desaturase

This part encodes a Δ6 desaturase derived from Synechocystis sp PCC 6803. The enzyme is a membrane-bound protein able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1, Δ9). This converts the substrate into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that feeding oleic acid to transformed E. coli, the bacterial membrane composition changes. Linoleic acid is observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None