Plasmid_Backbone
pSB8K15FRT
Part:BBa_K864022
Designed by: Erik Lundin Group: iGEM12_Uppsala_University (2012-09-24)
Low copy BioBrick temperature sensitive standard vector
pSB8K15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30’C but stop replicating at 42’C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711
Illegal NheI site found at 2425
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3717 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3711 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3711
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3711
Plasmid lacks a suffix.
Illegal XbaI site found at 3726
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2319 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
[edit]
Categories
Parameters
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/low
//plasmidbackbone/operation
//plasmidbackbone/version/5
//plasmidbackbone/copynumber/low
//plasmidbackbone/operation
//plasmidbackbone/version/5
chassis | |
copies | ~5 |
mcs | BioBrick |
origin | pSC101ts |
resistance | K |