Plasmid_Backbone
pSB8K15FRT

Part:BBa_K864022

Designed by: Erik Lundin   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 22:13, 24 September 2012 by Registry (Talk | contribs)

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Low copy BioBrick temperature sensitive standard vector

awd

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3711
    Illegal NheI site found at 2425
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3711
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3711
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3711
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3726
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/low
//plasmidbackbone/operation
//plasmidbackbone/version/5
Parameters
chassis
copies~5
mcsBioBrick
originpSC101ts
resistanceK