Device
OmpA-HIVcl

Part:BBa_K743011

Designed by: Bryon Silva   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 16:00, 22 September 2012 by Juan Alamos (Talk | contribs)

OmpA + HIV protease cleavage site secretion system

This device is designed for the further incorporation of any protein coding sequence downstream the HIV cleavage site. Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease.

If any polypeptide sequence is fused to the 3´ end of the HIV cleavage site DNA sequence, it will protrude from the outer membrane where it will be cleaved from the ompA domain by HIV protease.

It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP and a spider silk monomer. You can check the results HERE LINK!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
[edit]
Categories
Parameters
None