Composite
psb1C3_int

Part:BBa_K743009

Designed by:   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 23:45, 22 September 2012 by Juan Alamos (Talk | contribs)

psb1C3_IntKR recombination plasmid with RFP under psaAB promoter

The psb1C3_IntKR recombination plasmid allows the neutral integration of any desired sequence into Synechocystis PCC6803 chromosome. Composite part BBa_K743009 is designed for the expression of RFP under the control of the Synechocystis psaAB operon promoter.

Placas syne.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2657
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2657
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2657
    Illegal XhoI site found at 3154
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2657
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2657
    Illegal AgeI site found at 1270
    Illegal AgeI site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


[edit]
Categories
Parameters
None