Composite

Part:BBa_K929107

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)
Revision as of 12:13, 24 September 2012 by MariaK (Talk | contribs)

Anti-GFP, IgG1 Fc with TEV site, TMD, mCherry, framed by LoxP

Anti-GFP, IgG1 Fc with TEV site, TMR, mCherry, framed by LoxP
[[Image:|300px]]
BioBrick Nr. BBa_929107
RFC standard RFC 25
Requirement pSB1C3
Source Gene synthesis by GeneArt
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


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The antibody construct consists of two major building blocks represented by the actual antibody unit ( BBa_K929102) and the switchable membrane anchoring region (BBa_K929103). Both elements guarantee the eligibility and the easy handling of the construct and are optimized for expression in CHO cells. The antibody unit is represented by the human Ig kappa chain V-I region signal peptide (UniProt: P01601), the anti-GFP Nanobody (PDB: 3OGO) and the Fc region (UniProt: P01857). TEV protease recognition site, 2 LoxP sites, the B-cell receptor transmembrane domain (modified BBa_K157010) and the mCherry reporter display the switchable membrane anchoring region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None