Part:BBa_K763003

pGlnA + Gene encoding ZsYellow1

When there is no ammonia in the media the RNA-polimerase binds to RpoN Promoter and the transcription starts. Why?

In gram-negative bacteria, transcriptional activation in response to some external stimuli (absence of nitrogen, for example) often involves the alternative sigma factor, Sigma 54. This factor, also called RpoN or Sigma N, was originally identified as the sigma factor for nitrogen-controlled genes. Sigma 54 works in conjunction with members of the NtrC (Nitrogen regulatory protein C) superfamily of transcriptional activators.

“RpoN Promoter” contains a binding site which is recognized by the RNA-polimerase when Sigma 54 is binding to this protein. The polimerase binds to RpoN promoter which is present on genes/operons whose products minimize the slowing of growth under nitrogen-limiting conditions (aprox. 2% of the E. coli genome appears to be under NtrC control).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 689
  
• 1000
  COMPATIBLE WITH RFC[1000]