Reporter

Part:BBa_K763002

Designed by: Pedro Luis Dorado Morales   Group: iGEM12_Valencia_Biocampus   (2012-09-07)
Revision as of 09:38, 7 September 2012 by Registry (Talk | contribs)

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pNirB + Gene encoding ZsGreen1

When our bacteria are growing in absence of oxygen, ZsYellow1 gets expressed. Why?

FNR, is a DNA binding protein that regulates a large family of genes involved in cellular respiration and carbon metabolism during conditions of anaerobic cell growth. That is because FNR proteins are sensitive to little fluctuations in environmental oxygen concentration (FNR is believed to contain a redox/O2-sensitive element for detecting the anaerobic state). These proteins recognise a DNA target consisting of an inverted repeat: TTGATN1N2N3N4ATCAA (where N 1-4 represents a non-conserved tetrad, NCT).

Then, when there is no oxygen in the media, FNR proteins bind to promoter and the transcription is activated

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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