Plasmid

Part:BBa_K802003

Designed by: Sandu Ioana   Group: iGEM12_Lyon-INSA   (2012-09-20)
Revision as of 01:22, 24 September 2012 by Pishki (Talk | contribs) (→‎Characterization)

Shuttle vector for E. coli and B. subtilis

The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low plasmid copy number in B. subtilis and a high copy number in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6506
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6506
    Illegal NheI site found at 3377
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6506
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6506
    Illegal XbaI site found at 6521
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3203
    Illegal BsaI.rc site found at 5190


Characterization

Transformation yield

In the NM522 E.coli strain the average yield was 3,7XE06 cells/ DNA µg.

Antibiotic resistance

Sequencing of the modified regions

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Parameters
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