Part:BBa_K802005:Design
iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker was especially designed to contain the iGem restriction sites in the right order. It was obtained by the hybridization of two primers:
Forward: (5’)aattcgcggccgcttctagagaatactagtagcggccgctgcaga(3’)
Reverse: (5’)agcttctgcagcggccgctactagtattctctagaagcggccgcg(3’)
The percentage of the hybridization of the two primers is 91%. The only difference between the two consists in 8 nucleotides: 4 at the 5’end in the case of the Forward primer (which represents the sticky end compatible with a digested HindIII site) and the other 4 at 3’end in the case of the Reverse primer (which represents the sticky end compatible with a digested EcoRI site).
Source
The linker was obtained by the hybridization of two synthesized primers.