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Part:BBa_K802005:Design

Designed by: Sandu Ioana   Group: iGEM12_Lyon-INSA   (2012-09-21)
Revision as of 08:20, 21 September 2012 by Registry (Talk | contribs)

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iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning sites of the linker are EcoRI and HindIII (there is no need to digest the linker, the two extremities are sticky ends). Between these sites there are the other 3 iGEM enzyme restriction sites: XbaI, SpeI, PstI.


Source

The linker was obtained by the hybridization of two synthesized oligos.

References