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Other
Part:BBa_K802005:Design
Designed by: Sandu Ioana Group: iGEM12_Lyon-INSA (2012-09-21)
iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cloning sites of the linker are EcoRI and HindIII (there is no need to digest the linker, the two extremities are sticky ends). Between these sites there are the other 3 iGEM enzyme restriction sites: XbaI, SpeI, PstI.
Source
The linker was obtained by the hybridization of two synthesized oligos.