Regulatory
B-M

Part:BBa_K774000:Experience

Designed by: NRP-UEA-Norwich   Group: iGEM12_NRP-UEA-Norwich   (2012-06-25)
Revision as of 16:40, 25 June 2012 by Rkelwick (Talk | contribs) (→‎Applications of BBa_K774000)

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Applications of BBa_K774000

A hybrid of the PyeaR promoter (BBaK216005) and the E9-ns2 CArG promoter (Ref:1). Two versions will be synthesised, with the promoters altering their position in relation to the 5’-end of the sequence.

To provide additional restriction enzyme sites that may become useful during later cloning steps, BamHI, HindIII and NdeI have been added between the 2 promoters.

It is envisaged that any open reading frame (e.g. RFP or GFP) will be cloned “downstream” (i.e. at the 3’-end) of these promoter sequences.


Ref:1

Scott, S.D., Joiner, M.C. & Marples, B., 2002. Optimizing radiation-responsive gene promoters for radiogenetic cancer therapy. Gene therapy, 9(20), p.1396-402. Available at: http://www.ncbi.nlm.nih.gov/pubmed/12365005 [Accessed June 24, 2012].

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