Composite
Part:BBa_K808032
Designed by: Marie Burghard, Jascha Diemer, Philipp Rottmann, Rene Sahm, Arne Wehling Group: iGEM12_TU_Darmstadt (2012-09-04)
Arabinose regulated RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
This is a composite of 2 different BioBricks formed by a standard assembly, forming a scar in between. It contains the following units: AraC-AraO2-AraO1-AraPromo(PBAD-RBS-PhoA-His6tag-pNBEst13-Myctag-EstA which is similiar to BBa_K808000 & BBa_K808030 It is an operon, being induced by adding 0.02% to 0.5% of L-Arabinose. It is made for regulating the cell surface expression of our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2918
Illegal NgoMIV site found at 3056
Illegal NgoMIV site found at 3596
Illegal NgoMIV site found at 3965
Illegal AgeI site found at 979
Illegal AgeI site found at 2463 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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Categories
Parameters
//cds/enzyme
control | |
protein |