Composite
psb1C3_int

Part:BBa_K743008

Designed by: Bernardo Pollak, Isaac Nuñez   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 23:42, 22 September 2012 by Juan Alamos (Talk | contribs)

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803

This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis

Gel UC Chile.jpg Verification Colony PCR

Gel uc chile.jpg psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1758
    Illegal XhoI site found at 2255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


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Categories
Parameters
None