Generator
Part:BBa_K743015:Design
Designed by: Bernardo Pollak Group: iGEM12_UC_Chile (2012-09-22)
after dusk -> LuxAB luciferase (Vibrio fischerii)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 693
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 521
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2080
Design Notes
This part was made by Gibson assembly, therefore it has no scars
Source
Pta promoter was amplified from genomic Synechocystis DNA, you can find a biobrick version of this promoter at blahblahblah. LuxAB genes were obtained from the Luxbrick