Regulatory

Part:BBa_K733001:Design

Designed by: GU, Bida; SUN Fei   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 16:18, 22 September 2012 by Wyq.hkust (Talk | contribs) (→‎Design Notes)

Ptms - a constitutive promoter in E. coli and B. subtilis.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

 We first obtain the sequence of this part from http://dbtbs.hgc.jp/. Then we design two single strand oligonucleotides (primers) and use annealing and extension to get our intended promoter.

The sequences of these two primers are: Forward primer: 5’-CATGAAGTCTCCTTGAAATCAGAAGATATTTAGGATATATTTTTCTATGGAT–3’(Prefix not shown) Reverse primer: 5’–CAATATCCCTTTTATCCATAGAAAAATATATCCTAAATATCT–3’ (Suffix not shown)

Source

Not available at this moment.

References