Part:BBa_K802000
Lysostaphin generator for B. subtilis
This part associates the Bacillus subtillis Constitutive Promoter (PVeg) with the lysostaphin gene. Lysostaphin is a bacterial biocide isolated from Staphylococcus simulans and which specifically cleaves the pentaglycine cross bridges found in the staphylococcal peptidoglycan. It contains the necessary RBS to work. With this part, Bacillus subtillis strains cause the lysis of Staphylococcus aureus cells.
Characterization
Following results show that this part enables to B. subtillis 168 strains to kill the S. aureus and epidermidis cells.
In our plasmid collection, this part is named pBK23 in the backbone Chloramphenicol and pBK28 in the shuttle vector E. coli – B. subtillis (Part BBa_K802003). The corresponding negative control is the shuttle vector (pBK25 in our collection). We worked with the plasmid pBK28 for the tests and we tried two different genetic backgrounds: the strain NM522 to make test in E. coli and the strain Bs 168 to make test in Bacillus subtillis.
In you have any question on the following experiments, don’t forget that all the informations relative to our strains, plasmids and protocols are on our wiki notebook.
===Usage and Biology=== Sequence and Features
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