Part:BBa_K818000:Design

Integration vector for Bacillus subtilis derived from pSac-Cm

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5207
  Illegal XbaI site found at 5218
  Illegal SpeI site found at 1
  Illegal PstI site found at 12
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5207
  Illegal SpeI site found at 1
  Illegal PstI site found at 12
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5207
  Illegal BamHI site found at 180
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5207
  Illegal XbaI site found at 5218
  Illegal SpeI site found at 1
  Illegal PstI site found at 12
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 5207
  Illegal XbaI site found at 5218
  Illegal SpeI site found at 1
  Illegal PstI site found at 12
  Illegal NgoMIV site found at 4124
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2051
  Illegal SapI.rc site found at 3133
  

Design Notes

This part backbone plasmid design was derived from pSac-Cm Bacillus subtilis backbone plasmid. It has a terminator in its multiple cloning site and red fluorescent protein between the prefix and suffix sequences. It will integrate to B. subtilis chromosome via double recombination from the plasmid and chromosomal sacA sequences.

Source

Molecular Genetics Group at the University of Groningen, the Netherlands.

References

Becker E, Herrera NC, Gunderson FQ, Derman AI, Dance AL, Sims J, Larsen RA, Pogliano J. DNA segregation by the bacterial actin AlfA during Bacillus subtilis growth and development. EMBO J. 2006 Dec 13;25(24):5919-31. Epub 2006 Nov 30.