Generator
Part:BBa_K914007:Design
Designed by: Denis Samuylov Group: iGEM12_Paris_Bettencourt (2012-09-20)
Meganuclease I-SceI controlled by pBad
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.
Source
Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).