Coding

Part:BBa_K861150

Designed by: Xian Xia   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 06:05, 16 September 2012 by Shinkwee (Talk | contribs)

GalF, putative regulatory subunit for GalU

GalF is a predicted subunit of a GalU/GalF protein complex involved in colanic acid building blocks biosynthesis. While Glucose-1-phosphate uridylyltransferase(GalU), also referred to as UDP-glucose pyrophosphorylase or UGPase, catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP, the GalF is also essential for the formation and the function of the complex. The original GalF gene contains PstI restriction site. This part has already been point mutated (The T at 138bp was mutated to A).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


[edit]
Categories
Parameters
n/aGalF, putative regulatory subunit for GalU