Composite
psb1C3_int

Part:BBa_K743006

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 22:34, 20 September 2012 by Registry (Talk | contribs)

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psb1C3_IntK recombination plasmid for Synechocystis PCC6803

This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by UC_Chile 2012 team to succesfully transform Synechocystis [http://2012.igem.org/Team:UC_Chile]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1774
    Illegal XhoI site found at 2271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


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Categories
Parameters
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