Signalling

Part:BBa_K750007

Designed by: Sifan Wang,Ruosang Qiu,Shuqin Hu,Zhao Ma,Yunxin Long   Group: iGEM12_XMU-China   (2012-09-19)
Revision as of 09:10, 19 September 2012 by Registry (Talk | contribs)

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LuxR->LuxPR->GFP

This part contains part9, LuxPR and RBS1.0 GFP TT. When arabinose is added, the LuxR protein will be produced. And when this part is ligated with part4(or 5,6,7,8), the GFP will express because of the influence of quorum sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1080
    Illegal BsaI.rc site found at 1807


[edit]
Categories
Parameters
controlK206000
device_typesignalling
n/aLuxR->LuxPR->GFP
proteinsLuxR