Regulatory

Part:BBa_K731500

Designed by: Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)
Revision as of 12:37, 17 September 2012 by F.Guzz (Talk | contribs)

[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

This composite part consist of BBa_K731300 part (lacI-lacIq) and a tac promoter followed by a lac operator in the forward direction. It is a ready to use composite part and it allows the expression of a gene of interest with a strong E.coli promoter and under the control of IPTG. The lac operator decreases basal levels of expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None