Coding
Part:BBa_K929001:Design
Designed by: Potsdam Bioware 2012 iGEM Group: iGEM12_Potsdam_Bioware (2012-09-16)
AID without NES, with NLS and Kozak sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 108
Illegal SapI site found at 209
Design Notes
Primer (forward) with XbaI recognition site, kozak consensus sequence, NLS: ATCTAGAGCCGCCACCATGGGACCCAAGAAGAGGAAGGTGATGGACAGCCTCTTGATGAACCGGAGG
Primer (reverse, complement)with AgeI, SpeI and PstI recognition site: GCCTGCAGCGGCCGCTACTAGTATTAACCGGTGGGCAAAAGGATGCGCCGAAGC
Source
AID BioBrick (BBa_K103001) and self-designed primers