Terminator

Part:BBa_K731722

Designed by: Anna Depetris, Giacomo Giacomelli   Group: iGEM12_UNITN-Trento   (2012-08-23)
Revision as of 10:21, 15 September 2012 by Anna.d (Talk | contribs)

Terminator.png

biologyrrnBT1
directionForward
forward_efficiency0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac)

T1 terminator from E. coli rrnB

As many different teams had problems extracting BBa_B0010 from the Registry and cloning it, this is the same part newly submitted, confirmed by sequencing and by experience...ready to use!

The characterization of this terminator has been done both with T7 and E. coli RNA polymerases using BBa_K731700 and BBa_K731710 by the 2012 Trento iGEM team.

Examples of Secondary Structures

B0010 1.png B0010 2.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
//rnap/bacteriophage/t7
//terminator/single
Parameters
biologyrrnBT1
directionForward
forward_efficiency0.976 +/- 0.005 with T7 RNAP (T7 promoter) 0.985 +/- 0.001 with E. coli RNAP (Ptac)