Composite
araC-CysE

Part:BBa_K731020

Designed by: Jason Fontana   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 09:03, 23 August 2012 by Jasonfweb (Talk | contribs)

Inducible araC-pBAD promoter regulating WT CysE

The CysE gene (K731000) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None