Regulatory
Pcar

Part:BBa_K861171

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 06:26, 16 September 2012 by Shinkwee (Talk | contribs)

Pcar, synthetic promoter repressed by CRP

This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. Compared to PI (BBa_K861170), the binding of CRP and the promoter is stronger and the promoter is weaker due to the change of sequences in -10 region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aPcar, synthetic promoter repressed by CRP