Plasmid_Backbone
pSBBs1C

Part:BBa_K823021

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-16)
Revision as of 14:19, 11 September 2012 by Dexios (Talk | contribs)

pSBBs1C-lacZ (lacZ reporter vector for B. subtilis)

This part is a reporter vector for Bacillus subtilis. It integrates at the amyE locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for B.subtilis. The multiple cloning site is followed by a lacZ gene for reporter assays. This backbone is a BioBricked version of the B. subtilis vector pAC6.

This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke et al.]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 9777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 9771
    Illegal BglII site found at 8313
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 9771
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 9771
    Plasmid lacks a suffix.
    Illegal XbaI site found at 9786
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4562
    Illegal NgoMIV site found at 6888
    Illegal NgoMIV site found at 8688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4738
    Illegal SapI.rc site found at 5820


[edit]
Categories
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/libraryscreening/promoter
Parameters
chassisE.coli and B. subtilis
copiesintegrative in B. subtilis
originpAC6
resistanceA(E. coli) C(B. subtilis)