Coding

Part:BBa_K861100:Experience

Designed by: Xian Xia   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 11:46, 15 September 2012 by Nanhai (Talk | contribs) (→‎Applications of BBa_K861100)

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Applications of BBa_K861100

As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.


  • Antisense CCTGCAGTACTAGTATCATTGTTGAGCCAAAGCCTG

  • Sense　　　CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG

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    UNIQ66c745bfbf09679c-partinfo-00000000-QINU UNIQ66c745bfbf09679c-partinfo-00000001-QINU