Composite
Part:BBa_K731520
Designed by: Francesco Guzzonato Group: iGEM12_UNITN-Trento (2012-08-22)
IPTG inducible GFPmut3b
This is a construct carrying the sfGFP gene with low-efficiency RBS, under lac operator control.
lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.Low, constitutive levels of transcription can be seen anyhow.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1910
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Categories
Parameters
None |