Regulatory

Part:BBa_K731500

Designed by: Francesco Guzzonato   Group: iGEM12_UNITN-Trento   (2012-08-22)
Revision as of 14:35, 22 August 2012 by Registry (Talk | contribs)

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[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward

This is E.coli lactose-inducibile promoter.

lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription. Low, constitutive levels of transcription can be seen anyhow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None