Composite

Part:BBa_K731400:Design

Designed by: Andrea Tassinari   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 14:31, 23 August 2012 by Tassa (Talk | contribs) (→‎Design Notes)

IPTG inducible Cysteine desulfhydrase (CysDes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed so that LacI transcription/translation does not affect the transcription/translation of CysDes. In other words LacI+lacIq promoter are in the reverse direction. Tac promoter + Lac operator + CysDes are in the forward direction.

Source

LacI+LacIq promoter comes from pET-21b plasmid. CysDes gene comes from the genome of Treponema denticola.

References