Composite
araC-CysEm

Part:BBa_K731030

Designed by: Jason Fontana, Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 08:08, 23 August 2012 by Jasonfweb (Talk | contribs)

Inducible araC-pBAD promoter regulating M256I CysE

The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731200), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None