Composite
CysE-sfGFP

Part:BBa_K731040:Design

Designed by: Jason Fontana, Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-21)
Revision as of 08:10, 21 August 2012 by Registry (Talk | contribs)

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Inducible araC-pBAD promoter regulating a sfGFP-tagged M256I CysE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2075


Design Notes

Primers for Gibson's Assembly were designed to add a linker before sfGFP and remove the stop codons from CysE M256I.


Source

K731030

References