Protein_Domain

Part:BBa_J176132:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-10-28)
Revision as of 18:59, 1 August 2012 by Kahaynes (Talk | contribs) (→‎Design Notes)

PLrigid4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
    Illegal PstI site found at 95
    Illegal PstI site found at 110
    Illegal PstI site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Synthesized by GenScript; cloned into the pUC57 vector.
  • The PLrigid4 DNA sequence is not simply four tandem copies of the PLrigid DNA sequence. The sequence was edited manually to reduce DNA repetitiveness.

Source

TBA

References