RNA

Part:BBa_J100085

Designed by: Caroline Vrana   Group: Campbell M Lab   (2012-07-13)
Revision as of 13:01, 13 July 2012 by Registry (Talk | contribs)

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short CRISPR sequence with GFP target spacer

The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats) acts as a bacterial immune system. This sequence is a synthetic CRISPR system used in E.coli. It includes a short 30 bp sequence that targets the GFP gene (E0040). It is cloned into the plasmid pSB1K8.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 220
    Illegal XbaI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 221
    Illegal PstI site found at 235
    Illegal NotI site found at 6
    Illegal NotI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 221
    Illegal XbaI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 221
    Illegal PstI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None