Regulatory

Part:BBa_J100065:Design

Designed by: Rebecca Evans   Group: Campbell M Lab   (2012-06-11)
Revision as of 20:49, 11 June 2012 by Registry (Talk | contribs)

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Synthetic Riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal NotI site found at 7
    Illegal NotI site found at 201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 187


Design Notes

Because the riboswitch must be directly beside the gene of interest (the start codon is used in the folding of the riboswitch), we added a BsaI recognition site. This allows the riboswitch to be connected directly to a gene of interest using the Golden Gate Assembly method (if the first nucleotide after the start codon of the gene of interest is a C, we made it for use with superfolder GFP).


Source

http://www.ncbi.nlm.nih.gov/pubmed?term=Synthetic%20Riboswitches%20That%20Induce%20Gene%20Expression%20in%20Diverse%20Bacterial%20Species

References