Regulatory
HPK

Part:BBa_J176028:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 22:50, 8 December 2011 by Kahaynes (Talk | contribs) (→‎Design Notes)

HPK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Created by Kevin Shee (undergraduate, Pam Silver's lab, 2010)
  • PCR-cloned from the pmirGLO plasmid (Promega)
  • Primers add XbaI upstream and SpeI/NotI/PstI downstream
  • Two PstI sites mutated via site-directed mutagenesis (see sequence annotation)

Source

  • pmirGLO Dual-Luciferase miRNA Target expression vector (Promega)


References