Regulatory

Part:BBa_J153001

Designed by: Daniel Camsund / Hsinho Huang   Group: Lindblad Lab   (2012-02-03)
Revision as of 11:54, 3 February 2012 by Daniel Camsund (Talk | contribs) (New page: == ''Ptrc1O'' == P''trc1O'', or the ''trc'' promoter, is a hybrid of the ''Escherichia coli trp'' promoter and the ''lacUV5'' promoter (Brosius etal 1985) [http://www.ncbi.nlm.nih.gov/pu...)

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Ptrc1O

Ptrc1O, or the trc promoter, is a hybrid of the Escherichia coli trp promoter and the lacUV5 promoter (Brosius etal 1985) [http://www.ncbi.nlm.nih.gov/pubmed/2579077].

It contains the lac O1 operator (proximal to the core promoter) that allows for LacI repression and subsequent induction by IPTG.


Usage

It was characterized with a GFP reporter construct (Part:BBa_I13504) carried on the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) to be stronger than both the lacZYA operon promoter (Part:BBa_R0010) (ca 35% stronger) and a cI-based TetR-repressible promoter (Part:BBa_R0040) (ca 320 % stronger) in E. coli DH5alpha. Characterization in the cyanobacterium Synechocystis sp. PCC 6803 revealed strong fluorescence from the Ptrc1O-driven construct but no detectable signal from the “Plac” construct and only a weak signal from the “PtetR” construct. Repression of Ptrc1O by LacI in Synechocystis was very modest (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988].

The Ptrc2O promoter (Part:BBa_J153002) is of similar strength as Ptrc1O but significantly more repressed by LacI in Synechocystis. However, there are problems inducing this promoter to its full strength, limiting its usability.

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