Plasmid_Backbone

Part:BBa_J176124:Design

Designed by: Karmella Haynes, Andrew Younger   Group: Haynes Lab   (2011-10-22)
Revision as of 21:12, 19 December 2011 by Kahaynes (Talk | contribs) (→‎Design Notes)

pcDNA3.1+ zeo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NotI site found at 292
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal BglII site found at 12
    Illegal BamHI site found at 1438
    Illegal XhoI site found at 299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 266
    Illegal XbaI site found at 305
    Illegal SpeI site found at 249
    Illegal PstI site found at 271
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 1887
    Illegal NgoMIV site found at 1948
    Illegal NgoMIV site found at 2213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3738
    Illegal SapI site found at 2658


Design Notes

  • Built by Andrew Younger (Pam Silver lab undergrad intern)
  • An EM7-Zeocin resistance-polyA fragment (cut with BsmBI/ BstBI) was used to replace the puromycin region of the pcDNA3.1+ deltaCMV vector (No part name specified with partinfo tag.