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Plasmid_Backbone

Part:BBa_J176127:Design

Designed by: Brady Laughlin   Group: Haynes Lab   (2011-10-24)
Revision as of 20:52, 19 December 2011 by Kahaynes (Talk | contribs)

V0120


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NotI site found at 107
    Illegal NotI site found at 247
    Illegal NotI site found at 1832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal BglII site found at 1015
    Illegal BamHI site found at 318
    Illegal BamHI site found at 1021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1825
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NgoMIV site found at 4648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1571
    Illegal BsaI.rc site found at 3216
    Illegal SapI site found at 2133


Design Notes

  • This part was designed by Caroline Ajo-Franklin (Pam Silver lab postdoc). V0120 is a high-copy version of V0002. See <aprtinfo>BBa_J63009</partinfo> for more information.


Source

TBA

References