Part:BBa_J176013

VP64

Tetrameric VP16 transcription activator domain

Usage and Biology

The VP64 Activation Domain Module

• Chassis: mammalian cells
• Mammalian expression vector required
• Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
• VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain for the best results
  

VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447, DALDDFDLDML) connected with glycine-serine linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool, which pre-dates fancy terms like "synthetic biology." See the References section for some history on VP16 and VP64.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]