Protein_Domain
VP64

Part:BBa_J176013

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)
Revision as of 02:48, 22 November 2011 by Kahaynes (Talk | contribs) (→‎Characterization)

VP64

Tetrameric VP16 transcription activator domain

Usage and Biology

  • Chassis: mammalian cells
  • Mammalian expression vector required
  • Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression
  • VP64 doesn't do much as a free-floating domain. Fuse it to a DNA binding domain


VP64 is a transcriptional activator composed of four tandem copies of VP16 (Herpes Simplex Viral Protein 16, amino acids 437-447, DALDDFDLDML) connected with glycine-serine linkers. When fused to another protein domain that can bind near the promoter of a gene, VP64 acts as a strong transcriptional activator. This module is a classic molecular biology tool, which pre-dates fancy terms like "synthetic biology." See the References section for some history on VP16 and VP64.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/eukaryote/human
//proteindomain/activation
Parameters
chassismammalian cells
functiontranscription
uniprotP06492